The cell line optimization 96-well Nucleofector® kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System

With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment. Using one 96-well Nucleocuvette® plate and our pmaxGFP® vector as positive control, our three cell line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls.

Applications: Determination of Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.