Monocytes are found in the circulating peripheral blood where they make up 10 - 20% of the total mononuclear cell population.

• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

Monocytes are characterized by their phenotypic expression of CD14, and thus, are commonly called CD14+ monocytes. They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages, and can differentiate into dendritic cells with potent antigen-presenting capability.

Cells You Can Count on to Perform:
Lonza Human Peripheral Blood CD14+ Monocytes are isolated from peripheral blood of screened, healthy, untreated (non-mobilized peripheral blood) donors. Briefly, mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood. The MNC population is then further purified via positive immunomagnetic selection directed against CD14. Cryopreserved CD14+ Monocytes are guaranteed to express CD14 and will contain either ≥10, 20, or 40 million viable cells per ampoule depending upon the product ordered.

Choices in Cell Type and Tissue Source:
Hematopoietic and immune research requires a variety of donors and cell types. Lonza offers many different peripheral blood cell types from a variety of donors, including PBMCs and dendritic cells, among others. In addition, if you are interested in exploring the differences between monocytes in different maturation states or from different tissues, bone marrow mononuclear cells available.

Monocyte Phenotype: RPMI + 10% Fetal Bovine Serum + 2 mM L-glutamine + 10 ng/ml rHU M-CSF (optional)
Macrophages: RPMI +10% Fetal Bovine Serum + 2 mM L-glutamine + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids + 25 ng/ml rHU M-CSF
Dendritic Cells: LGM-3® Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza CD14+ monocytes are not routinely quality control tested for macrophage or dendritic cell differentiation and differentiation is not guaranteed under the cell warranty. Additional factors including extracellular matrixes or culturing conditions may be required to produce differentiation.