MabSelect™ VL protein L resin in HiScreen™ chromatography columns. A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability.

• High dynamic binding capacity for efficient capture of target molecules
• Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
• Provides good resolution for product-related impurities in the capture of bispecific antibodies
• MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to our Capto™ L chromatography resin
• This higher capacity provides productivity advantages in research and biomanufacturing
• The MabSelect™ VL protein L resin can withstand cleaning with 0.1 M NaOH

As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.

HiScreen™ columns are convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development. MabSelect™ VL protein L resin is also available in 1 and 5 ml HiTrap™ prepacked columns, bulk resins in different pack sizes, PreDictor™ RoboColumn® minicolumns, and ReadyToProcess™ columns for purifying protein as well as screening and process evaluation.