To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Cat. no.:77213-060
Specifications
About this item
Human TRH-R ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TRH-R in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 1 to 16 ng/ml
- Sensitivity: 0.029 ng/ml
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human TRH-R ELISA kit (A312557) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TRH-R in An antibody specific for TRH-R has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TRH-R present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TRH-R antibody, which also binds the TRH-R present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TRH-R antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TRH-R antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TRH-R captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TRH-R in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.