To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:7.74 ng/L
- Target protein:Thyroid Hormone Receptor beta
- Description:Human Thyroid Hormone receptor beta ELISA kit
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:100 - 1600 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
- Cat. no.:77213-148
Specifications
About this item
Human Thyroid Hormone Receptor beta ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Thyroid Hormone Receptor beta in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 100 to 1600 ng/L
- Sensitivity: 7.74 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human Thyroid Hormone Receptor beta ELISA kit (A312895) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Thyroid Hormone Receptor beta in An antibody specific for Thyroid Hormone Receptor beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Thyroid Hormone Receptor beta present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Thyroid Hormone Receptor beta antibody, which also binds the Thyroid Hormone Receptor beta present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Thyroid Hormone Receptor beta antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Thyroid Hormone Receptor beta antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Thyroid Hormone Receptor beta captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Thyroid Hormone Receptor beta in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.