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Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Mouse
- Sensitivity:0.023 ng/ml
- Target protein:Spink7
- Description:Mouse Spink7 ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:1 - 16 ng/mL
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
Specifications
About this item
Mouse Spink7 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Spink7 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 1 to 16 ng/mL
- Sensitivity: 0.023 ng/ml
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Mouse Spink7 ELISA kit (A312945) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Spink7 in An antibody specific for Spink7 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Spink7 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Spink7 antibody, which also binds the Spink7 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Spink7 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Spink7 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Spink7 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Spink7 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.