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- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:OVGP1/OGP
- Description:Human OVGP1/OGP ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:150 - 2400 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:8.82 ng/L
- Regulatory status:RUO
- Cat. no.:77205-746
Human OVGP1/OGP ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human OVGP1/OGP in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 150 to 2400 ng/L
- Sensitivity: 8.82 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human OVGP1/OGP ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human OVGP1/OGP in serum, plasma or other biological fluids. An antibody specific for OVGP1/OGP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the OVGP1/OGP present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-OVGP1/OGP antibody, which also binds the OVGP1/OGP present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-OVGP1/OGP antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-OVGP1/OGP antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of OVGP1/OGP captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of OVGP1/OGP in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.