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Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Mouse
- Sensitivity:0.022 ng/ml
- Target protein:Factor VIII
- Description:Mouse factor VIII ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.75 - 12 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
Specifications
About this item
Mouse Factor VIII ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Factor VIII in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 0.75 - 12 ng/mL
- Sensitivity: 0.022 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse Factor VIII ELISA kit (A313514) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Factor VIII in serum, plasma or other biological fluids. An antibody specific for Factor VIII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Factor VIII present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Factor VIII antibody, which also binds the Factor VIII present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Factor VIII antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Factor VIII antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Factor VIII captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Factor VIII in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.