To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:0.092 ng/ml
- Target protein:Coxsackie Adenovirus Receptor/hCAR
- Description:Human coxsackie adenovirus receptor/hCAR ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:2 - 32 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
Specifications
About this item
Human Coxsackie Adenovirus Receptor/hCAR ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Coxsackie Adenovirus Receptor/hCAR in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 2 - 32 ng/ml
- Sensitivity: 0.092 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Coxsackie Adenovirus Receptor/hCAR ELISA kit (A310811) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Coxsackie Adenovirus Receptor/hCAR in serum, plasma or other biological fluids. An antibody specific for Coxsackie Adenovirus Receptor/hCAR has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Coxsackie Adenovirus Receptor/hCAR present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Coxsackie Adenovirus Receptor/hCAR antibody, which also binds the Coxsackie Adenovirus Receptor/hCAR present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Coxsackie Adenovirus Receptor/hCAR antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Coxsackie Adenovirus Receptor/hCAR antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Coxsackie Adenovirus Receptor/hCAR captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Coxsackie Adenovirus Receptor/hCAR in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.