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- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:Carbonic Anhydrase 4/CA4
- Description:Human Carbonic Anhydrase 4/CA4 ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:2 - 32 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Sensitivity:0.093 ng/ml
- Regulatory status:RUO
- Cat. no.:77174-022
Human Carbonic Anhydrase 4/CA4 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Carbonic Anhydrase 4/CA4 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human Carbonic Anhydrase 4/CA4 ELISA kit (A313547) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Carbonic Anhydrase 4/CA4 in serum, plasma or other biological fluids. An antibody specific for Carbonic Anhydrase 4/CA4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Carbonic Anhydrase 4/CA4 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Carbonic Anhydrase 4/CA4 antibody, which also binds the Carbonic Anhydrase 4/CA4 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Carbonic Anhydrase 4/CA4 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Carbonic Anhydrase 4/CA4 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Carbonic Anhydrase 4/CA4 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Carbonic Anhydrase 4/CA4 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.