This skills-building lab introduces fluorescence and its applications in biotechnology.

• Techniques: Micropipetting, serial dilutions, fluorescence detection
• Topics: Fluorescence, biotechnology, concentrations, scientific notation
• Time required: Can be completed in one 45-minute period
• Level: General high school through advanced high school

This lab serves as an introduction to the phenomenon of fluorescence and its applications in biotechnology. It also provides an engaging framework to develop foundational lab skills such as micropipetting and making serial dilutions, through which students create a standard curve to estimate unknown solute concentrations. Students will explore the properties of fluorescein, a commonly used indicator in biotechnology applications. By taking an inquiry-based approach, students will grow comfortable using indicator molecules to answer experimental questions. Go fluorescein glow!

Required equipment (one per lab group):
- Micropipettes and tips.
- P51™ Molecular Fluorescence Viewer or other blue light transilluminator (460 to 480 nm, e.g. blueBox or blueGel).

Storage notes:
- Reagents can be stored at room temperature, protected from light.
- Reagents are stable for 24 months under recommended conditions.

Note: Reagents are compatible with P51™ Molecular Fluorescence Viewer or other blue light transilluminator (460 to 480 nm, e.g. blueBox™ or blueGel™).