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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:46.875 pg/ml
- Target protein:Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP
- Description:Human cross linked C-terminal telopeptide of type I collagen/ICTP ELISA kit
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP ELISA Kit exhibits high specificity and excellent specificity for the detection of human Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP. No significant cross-reactivity or interference between Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:78.125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
- Cat. no.:76746-676
Specifications
About this item
Human Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Cross Linked C-terminal telopeptide of Collagen alpha-1(I) Chain/ICTP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 78.125 to 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP ELISA kit (A78277) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP Antibody, which binds the captured Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP captured in each well. The concentration of Cross Linked C-terminal Telopeptide of Type I Collagen/ICTP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.