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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:46.875 pg/ml
- Target protein:Syntaxin 16
- Description:Human syntaxin 16 ELISA kit
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human Syntaxin 16 ELISA Kit exhibits high specificity and excellent specificity for the detection of human Syntaxin 16. No significant cross-reactivity or interference between Syntaxin 16 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:78.125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
- Cat. no.:76738-366
Specifications
About this item
Human Syntaxin 16 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Syntaxin 16 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human Syntaxin 16 ELISA kit (A302776) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Syntaxin 16 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Syntaxin 16 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Syntaxin 16 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Syntaxin 16 Antibody, which binds the captured Syntaxin 16 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Syntaxin 16 captured in each well. The concentration of Syntaxin 16 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.