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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Rat
- Sensitivity:0.094 ng/ml
- Target protein:PLA2G1B
- Description:Rat PLA2G1B ELISA kit
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat PLA2G1B ELISA kit exhibits high specificity and excellent specificity for the detection of rat PLA2G1B. No significant cross-reactivity or interference between PLA2G1B and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
- Cat. no.:76743-092
Specifications
About this item
Rat PLA2G1B ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat PLA2G1B in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0.156 - 10 ng/ml
- Sensitivity: 0.094 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat PLA2G1B ELISA kit (A87155) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat PLA2G1B in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PLA2G1B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PLA2G1B present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PLA2G1B Antibody, which binds the captured PLA2G1B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PLA2G1B captured in each well. The concentration of PLA2G1B can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.