To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:46.875 pg/ml
- Target protein:CDK12
- Description:Human CDK12 ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human CDK12 ELISA kit exhibits high specificity and excellent specificity for the detection of human CDK12. No significant cross-reactivity or interference between CDK12 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:78.125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
Specifications
About this item
Human CDK12 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CDK12 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 78.125 - 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human CDK12 ELISA kit (A76291) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CDK12 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CDK12 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CDK12 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CDK12 Antibody, which binds the captured CDK12 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CDK12 captured in each well. The concentration of CDK12 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.