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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Rat
- Sensitivity:0.094 ng/ml
- Target protein:Aquaporin 5
- Description:Rat Aquaporin 5 ELISA kit
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat Aquaporin 5 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Aquaporin 5. No significant cross-reactivity or interference between Aquaporin 5 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
- Cat. no.:76740-712
Specifications
About this item
Rat aquaporin 5 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat aquaporin 5 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Rat Aquaporin 5 ELISA kit (A247193) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Aquaporin 5 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Aquaporin 5 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aquaporin 5 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-Aquaporin 5 antibody, which binds the captured Aquaporin 5 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Aquaporin 5 captured in each well. The concentration of Aquaporin 5 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.