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Specifications
- Assay duration:Multiple steps
- Assay Type:Competitive
- Reactivity:Rat
- Sensitivity:4.688 pg/ml
- Target protein:PACAP-27
- Description:Rat PACAP-27 ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Rat PACAP-27 ELISA kit exhibits high specificity and excellent specificity for the detection of rat PACAP-27. No significant cross-reactivity or interference between PACAP-27 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7.813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Regulatory status:RUO
Specifications
About this item
Rat PACAP-27 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat PACAP-27 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 7.813 - 500 pg/ml
- Sensitivity: 4.688 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat PACAP-27 ELISA kit (A74923) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat PACAP-27 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with PACAP-27 antigen. During the incubation, PACAP-27 present in the samples or standards competes with the fixed amount of immobilized PACAP-27 for binding sites on the Biotinylated Anti-PACAP-27 Antibody. The more PACAP-27 present in a sample or standard, the less Biotinylated Anti-PACAP-27 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-PACAP-27 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of PACAP-27 present in each sample or standard. The concentration of PACAP-27 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.