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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Rat
- Sensitivity:0.094 ng/ml
- Target protein:IGF1 Receptor
- Description:Rat IGF1 receptor ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat IGF1 Receptor ELISA kit exhibits high specificity and excellent specificity for the detection of rat IGF1 Receptor. No significant cross-reactivity or interference between IGF1 Receptor and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
Specifications
About this item
Rat IGF1 receptor ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat IGF1 receptor in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0.156 to 10 ng/ml
- Sensitivity: 0.094 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Rat IGF1 Receptor ELISA kit (A303761) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat IGF1 Receptor in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for IGF1 Receptor has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IGF1 Receptor present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IGF1 Receptor antibody, which binds the captured IGF1 Receptor present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IGF1 Receptor captured in each well. The concentration of IGF1 Receptor can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.