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Specifications
- Assay duration:Multiple steps
- Assay Type:Competitive
- Reactivity:Mouse
- Sensitivity:46.875 pg/ml
- Target protein:beta Crosslaps/beta CTx
- Description:Mouse beta crosslaps/beta CTx ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse beta Crosslaps/beta CTx ELISA kit exhibits high specificity and excellent specificity for the detection of mouse beta Crosslaps/beta CTx. No significant cross-reactivity or interference between beta Crosslaps/beta CTx and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:78.125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Regulatory status:RUO
Specifications
About this item
Mouse beta Crosslaps/beta CTx ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse beta Crosslaps/beta CTx in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 78.125 - 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse beta Crosslaps/beta CTx ELISA kit (A75978) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse beta Crosslaps/beta CTx in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with beta Crosslaps/beta CTx antigen. During the incubation, beta Crosslaps/beta CTx present in the samples or standards competes with the fixed amount of immobilized beta Crosslaps/beta CTx for binding sites on the Biotinylated Anti-beta Crosslaps/beta CTx Antibody. The more beta Crosslaps/beta CTx present in a sample or standard, the less Biotinylated Anti-beta Crosslaps/beta CTx Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-beta Crosslaps/beta CTx Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of beta Crosslaps/beta CTx present in each sample or standard. The concentration of beta Crosslaps/beta CTx can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.