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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:46.875 pg/ml
- Target protein:DNASE2
- Description:Human DNASE2 ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human DNASE2 ELISA Kit exhibits high specificity and excellent specificity for the detection of human DNASE2. No significant cross-reactivity or interference between DNASE2 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:78.125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
Specifications
About this item
Human DNASE2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human DNASE2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection Range: 78.125 - 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human DNASE2 ELISA kit (A247350) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human DNASE2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for DNASE2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the DNASE2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-DNASE2 antibody, which binds the captured DNASE2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of DNASE2 captured in each well. The concentration of DNASE2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.