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Specifications
- Assay duration:Multiple steps
- Assay Type:Competitive
- Reactivity:Dog
- Sensitivity:0.094 ng/ml
- Target protein:Prolactin/PRL
- Description:Canine prolactin/PRL ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Canine Prolactin/PRL ELISA Kit exhibits high specificity and excellent specificity for the detection of canine Prolactin/PRL. No significant cross-reactivity or interference between Prolactin/PRL and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Regulatory status:RUO
Specifications
About this item
Canine Prolactin/PRL ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of canine Prolactin/PRL in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Canine Prolactin/PRL ELISA kit (A247360) employs the competitive enzyme immunoassay technique for the quantitative measurement of canine Prolactin/PRL in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Prolactin/PRL antigen. During the incubation, Prolactin/PRL present in the samples or standards competes with the fixed amount of immobilized Prolactin/PRL for binding sites on the Biotinylated Anti-Prolactin/PRL Antibody. The more Prolactin/PRL present in a sample or standard, the less Biotinylated Anti-Prolactin/PRL Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Prolactin/PRL Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Prolactin/PRL present in each sample or standard. The concentration of Prolactin/PRL can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.