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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:0.188 ng/ml
- Target protein:UBE3A
- Description:Human UBE3A ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human UBE3A ELISA Kit exhibits high specificity and excellent specificity for the detection of human UBE3A. No significant cross-reactivity or interference between UBE3A and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.313 - 20 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
Specifications
About this item
Human UBE3A ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human UBE3A in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 0.313 to 20 ng/ml
- Sensitivity: 0.188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human UBE3A ELISA kit (A77475) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human UBE3A in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for UBE3A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the UBE3A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-UBE3A Antibody, which binds the captured UBE3A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of UBE3A captured in each well. The concentration of UBE3A can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.