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Cheetah™ HotStart Taq DNA Polymerase, Biotium
Cheetah™ HotStart Taq DNA Polymerase, Biotium
Catalog #: 89138-878
Supplier:  Biotium
CAS Number:  
Cheetah* Taq
Cheetah™ HotStart Taq DNA Polymerase, Biotium
Catalog #: 89138-878
Supplier:  Biotium
CAS Number:  
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Specifications

  • Hot start:
    Chemically modified
  • Description:
    Cheetah™ Hot Start Taq Polymerase
  • Format:
    Enzyme with/without buffer
  • Size:
    500 U
  • Storage conditions:
    Store at -10 to -35 °C
  • Processivity:
    Fast
  • Exonuclease activity:
    3’→5’
  • Concentration:
    5U/uL
  • Units:
    500
  • Cat. no.:
    89138-878
  • Supplier No.:
    29050

Specifications

About this item

A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.

  • Hot-start Taq polymerase
  • Modified using unique hot-start chemistry
  • Absolutely no activity at room temperature
  • Fast activation: takes less than 2 minutes of heating to activate
  • Very stable during storage

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

Under standard hot-start conditions (i.e., 94°C in pH 8-9 Tris), Cheetah™ Taq regains both the 3′-5′ polymerase and 5′-exonuclease activities within two minutes. Cheetah™ Taq is also superior to antibody-based hot-start DNA polymerases due to its complete suppression of enzyme activity at room temperature and full recovery of enzyme activity following activation.

AmpliTaq is a registered trademark of Thermo Fisher Scientific.

Ordering information: Kit contents Cheetah™ Taq, 5 U/uL, 100 uL (in buffer containing Tris-HCl pH 9.0, DTT, EDTA, KCl and glycerol), 10X Cheetah™ Taq Reaction Buffer (without Mg2+) 1.5 mL; 25 mM MgCl2, 1.5 mL
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