To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Description:SARS-CoV-2 rapid colorimetric LAMP assay kit
- Size:96 reactions
- Cat. no.:CA76447-118
Specifications
About this item
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.
- 30 minute isothermal protocol – no sophisticated lab equipment required
- Colorimetric, visual detection – ease of use, simple-to-read results
- UDG and dUTP included in the master mix for carryover prevention – reduced risk of contamination
- Optimized dual primer mix – enhanced detection and sensitivity
- Dual WarmStart formulation – room temperature setup
- Necessary controls included – verify assay performance for increase confidence in results
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes Loop-Mediated Isothermal Amplification (LAMP) to detect SARS-CoV-2 nucleic acid. The kit is available for research use only and includes WarmStart Colorimetric LAMP 2X Master Mix with UDG and a primer mix targeting the N and E regions of the viral genome. Controls are provided to verify assay performance, and include an internal control primer set and a positive control template. Guanidine hydrochloride has been found to increase the speed and sensitivity of the RT-LAMP reaction and is also included.
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.
This system provides a fast, clear visual detection of amplification based on the production of protons and the subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction. The decrease in pH produces a change in solution color from pink to yellow. The total protocol time is 30 minutes and only requires a simple heating device that can reach 65 °C.
A positive detection of the SARS-CoV-2 RNA sequence would be indicated by a yellow color (amplification occurred, protons released, pH-dependent color change from pink to yellow), while a negative result would be indicated by a pink color (no amplification occurred, no protons released, no color change).