The Vivapure® AdenoPACK™ adenovirus purification and concentration kits provides researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.

• Fast and easy virus purification: Purification typically completed in 2 hours; convenient over 10x faster alternative to CsCl density gradient
• Quantitative yields: In contrast to CsCl density gradient, the complete cell culture is used for virus purification and not only the viral pellet
• Flexible product range: Applicable from initial construct screening to large scale virus production
• Complete kit: Including filtration devices, AdenoPACK units for virus purification, Vivaspin and all buffers
• Low endotoxin levels: High cell viability and infection rates due to endotoxin levels of <0.025 EU/ml

Using AdenoPACK 20 are supplied in a spin column format for centrifuges. AdenoPACK 100 is a manually operated kit in a syringe filter format and AdenoPACK 500 is a pump-driven kit. For each sample volume, the most convenient handling method is provided for more convenience.

However, for even more convenience, protocols are provided for optionally running the virus purification with a peristaltic pump or with an infusion pump, in additional to detailed instructions for a manual operation supplied with the kit. The accessories needed for the operation with a pump are supplied as individual products.

AdenoPACK™ 100/100 RT, resulting in purifying virus particles of 1×10¹¹ - 10¹² per ml with infectivity ratio of 50 to 100. AdenoPACK™ 200/200 RTresulting in purifying virus particles of 1×10¹³ per ml with infectivity ratio of 20 to 50. AdenoPACK™ 500/500 RTresulting in purifying virus particles of 3×10¹³ per ml with infectivity ratio of 20 to 50.

*RT kit does not contain Benzonase® DNA-digesting enzyme.