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Specifications
- Antibody type:Primary
- Antigen name:Tumor necrosis factor receptor superfamily member 16
- Clonality:Monoclonal
- Clone:MC192
- Gene ID:Ngfr
- Host:Mouse
- Isotype:IgG1
- Reactivity:Rat
- Antigen symbol:TNR16
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 µg
- Epitope:Extracellular domain
- Form:Liquid
- Antigen synonyms:Low affinity neurotrophin receptor p75NTR|NGF receptor|p75 ICD|Gp80-LNGFR|Low-affinity nerve growth factor receptor
- Storage temperature:Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20° C for long-term storage. Store at 2…8° C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
- Shipping temperature:2…8° C
- Immunogen:N-octyl glucoside solubilized proteins from isolated PC12 cell plasma membranes were used as the immunogen (see Chandler et al. 1984).
- Purification:Protein G purified.
- Cat. no.:76333-988
Specifications
About this item
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR.
Applications: IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5ug/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20ug/mL) is recommended, unfixed cells.
Type: Primary
Antigen: TNR16
Clonality: Monoclonal
Clone: MC192
Conjugation: Atto488
Epitope: Extracellular domain
Host: Mouse
Isotype: IgG1
Reactivity: Rat