About this item
Engineer cells with a DNA program where gene expression can be manually controlled with temperature.
- Safety First! The kit uses a non-pathogenic strain of E. coli (K-12)
- Recommended for use with Amino Labs DNA Playground™ (available separately)
The Heat-It Kit™ lets you grow and engineer bacteria with a DNA plasmid that can be controlled with heat. Decide when you want the DNA program to turn on by choosing the temperature you grow the bacteria at. Turn the DNA program on and off to create tree ring patterns in the growth.
In this kit, students will: Make selective and non-selective agar, pour petri dishes, streak bacteria, make bacteria chemically competent, engineer bacteria using DNA plasmid (bacterial transformation), create positive and negative controls, incubate at different temperatures, view or analyze results.
Additional equipment required but not included: Microwave or hot-plate, and Amino Labs DNA Playground™ (In the absence of a DNA Playground™ you will also need an ice bath, hot water bath, thermometer, timer, and incubator).