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Specifications
- Size:2 ml
- Storage conditions:Store product at 2-8 °C. Addition of 0.05% sodium azide or other preservative to the equilibration buffer is recommended to inhibit microbial growth during storage. Do not freeze.
- Protein/peptide name:IgG
- Tested applications:Immunoassays
- Cat. no.:IC0855961
Specifications
About this item
Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.
IgG affinity gel comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of rabbit IgG from serum and other sources.
- The chromatography gel can be used for affinity purification of antibodies to rabbit IgG or to remove these antibodies from an antiserum preparation.
- With appropriate elution buffers, the gel may be re-used.
- Binding Capacity: 2.3 mg antibody/mL of gel
- The chromatography gel is in 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v).
- The antigen reacts with anti-rabbit IgG and may cross-react to antibodies of other species. Each mL of gel has a minimum binding capacity of 2.0 mg of antibody.
This antigen affinity gel is prepared by reaction of purified IgG to activated Sepharose® 4B gel. The washed gel is packaged as a ready-to-use column. The bed volume is 2 mL.
IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.