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Specifications
- Source:Human
- Species:Human
- Size:50 mg
- Storage conditions:Store unopened product at 2-8 °C. Store reconstituted material no longer than a few days at 2-8 °C unless sodium azide is added. For long-term storage, aliquot and freeze at –20 °C.
- Protein/peptide name:IgG
- Purity:Greater than 98% by SDS-PAE, purified antigen
- Concentration:Total Protein: 17.0 mg/mL, Volume: 30 mL
- Formulation:Lyophilized powder
- Tested applications:Immunoassays
- Cat. no.:IC0855908
Specifications
About this item
Product is the lyophilized powder of human IgG and buffer salts.
- This product is suitable for use as a positive control in antibody identification screening techniques, and is recommended as an ELISA coating antigen and control.
- Purified Human IgG is suitable for use as a positive control in antibody identification screening techniques, and is recommended as an ELISA coating antigen and control.
- Human IgG is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption.
- The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized from a 8.43 ml solution. No preservative has been added.
All blood products should be treated as potentially infectious. Source material from which this product was derived was found negative when tested in accordance with current FDA required tests. No known test methods can offer assurance that products derived from human blood will not transmit infectious agents. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 5 percent so that a minimum of 500 mg of human IgG is obtained upon reconstitution.
Human IgG is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 μm filter, vialed and lyophilized from a 30 ml solution. No preservative has been added.
Reconstitution: This product can be reconstituted to 10 - 25 mg/mL, as desired. Sterile water or 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3 can be used. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.