Human E-Cadherin AccuSignal ELISA Kit

• Natural and recombinant human E-Cadherin.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: NSO; Immunogen sequence: D155-I707

E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). dd 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. We recommend that each human E-Cadherin standard solution and each sample is measured in duplicate.