Human VCAM-1 AccuSignal ELISA Kit

• Natural and recombinant human VCAM-1.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: NSO; Immunogen sequence: F25 - E698

Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human VCAM-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human VCAM-1 standard solution and each sample is measured in duplicate.