Human IL1R1 AccuSignal ELISA Kit

• Natural and recombinant human IL1R1.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: NSO; Immunogen sequence: D21-T332

Interleukin 1 receptor, type I(IL1R1), also known as CD121a(Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2(IL1RL2), and interleukin 1 receptor-like 1(IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha(IL1A), interleukin 1 beta(IL1B), and interleukin 1 receptor antagonist(IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human IL1R1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human IL1R1 standard solution and each sample be measured in duplicate.