Anti-PARP1 (N-term ZF1) Antibody Set

• Set Contains:
• 200-401-GM8S - Rabbit Anti-PARP1 (N-term ZF1) purified antibody
• 009-001-GM8 - PARP1 (N-term ZF1) protein- an N-terminus His-Tag recombinant protein expressed in E.coli corresponding to a fragment of the human PARP1 zinc finger domain
• 611-103-122-0100 - Goat Anti-Rabbit IgG Peroxidase secondary antibody

PARP1 is the primary member of the poly(ADP-ribose) polymerase family, whose function is to signal DNA damage (and to recruit repair proteins) by PARylation. PARP1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. PARP1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-PARP1 (N-term ZF1) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.

Anti-PARP1 (N-term ZF1) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract. PARP1 (N-term ZF1) Control Protein analysis by SDS-PAGE resulted in a band at a MW ~13 kDa and estimated to be greater than 95% pure by Coomassie staining.