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Specifications
- Description:3-Desthiobiotin-GTP 5 mM
- Size:0.5 mol
- Cat. no.:CA103307-266
Specifications
About this item
Cappable-seq is a method, developed at NEB, for directly enriching the 5' ends of primary transcripts.
The method enables determination of transcription start sites at single base resolution. This is achieved by capping the 5' triphosphorylated end of RNA with Vaccinia Capping System and 3'-Desthiobiotin-GTP. The primary transcripts are then bound to Hydrophilic Streptavidin Magnetic Beads, washed and eluted with free biotin.
Supplied in: Ultrapure water as a Triethylammonium salt at pH 7.0
Molecular Formula: C₃₁H₅₂N₁₁O₁₉P₃
Molecular Weight: 975.74 g/mol
Extinction Coefficient: 260 = ~11700 Lmol⁻¹ cm⁻¹