About this item
Urease test broth is a differential medium for organisms, particularly members of the Enterobacteriaceae, on the basis of their ability to produce urease.
- Peptone and dextrose provide nutrients
- Reduced buffer content helps promote rapid growth
- Urease base is also supplied as a filter sterilized 10X concentrated solution for use in preparing urease test broth in the user’s laboratory
Urease test broth can be used for the identification of bacteria on the basis of urea utilization and it is particularly recommended for the differentiation of members of the genus proteus from those of salmonella and shigella in the diagnosis of enteric infections.
When organisms utilize urea, ammonia is formed during incubation, which makes the reaction of these media alkaline, producing a pink-red color. Consequently, urease production may be detected by the change in the phenol red indicator.
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