About this item
This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Type: Primary
Antigen: NCL
Clonality: Monoclonal
Clone: Clone 364-5 NCL/902
Conjugation: CF405S
Epitope:
Host: Mouse
Isotype: IgG's
Reactivity: Human
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Specifications
- Catalog No:
- 75966-488
- 75966-490
- Antigen name:
- Nucleolin
- Nucleolin
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Clone:
- 364-5 NCL/902
- 364-5 NCL/902
- Host:
- Mouse
- Mouse
- Gene ID:
- Unigene ID: 79110
- Unigene ID: 79110
- Isotype:
- IgG
- IgG