About this item
This MAb recognizes human 17-26 kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.
Type: Primary
Antigen: TNF
Clonality: Monoclonal
Clone: Clone TNF655
Conjugation: CF488A
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human
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Specifications
- Catalog No:
- 75976-916
- 75976-918
- Antigen name:
- TNF alpha
- TNF alpha
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Clone:
- TNF655
- TNF655
- Host:
- Mouse
- Mouse
- Gene ID:
- Unigene ID: 241570
- Unigene ID: 241570
- Isotype:
- IgG1 kappa
- IgG1 kappa