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Specifications
- Description:illustra Hot Start Master Mix
- Size:100 reactions
- Storage conditions:-20°C
- Cat. no.:CA11028-990
- Supplier No.:25-1500-01
Specifications
About this item
illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR.
- Recombinant hot start activator protein sequesters primers prior to PCR, effectively reducing non specific priming and primer-dimer formation
- Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment
- Avoids the risk of contamination from a mammalian source associated with hot start antibodies
- Convenient, ready to use mix saves time and reduces experimental variability
- Confidence in downstream applications; increased read length in sequencing applications
- Appropriate for any standard PCR reaction, such as multiplex PCR, and reactions exhibiting non specific amplification or primer-dimer formation
illustra™ Hot Start Master Mix combines high quality recombinant Taq DNA polymerase, a recombinant hot start activator protein, and nucleotides in a proprietary reaction buffer. This ready to use mix provides robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success. The mix does not contain Taq antibody and this eliminates the risk of contamination from a mammalian source. Also, since the polymerase is not chemically inactivated, there is no extensive pre-cycling heating step necessary; this reduces the chance of damaging precious DNA samples from heat-induced depurination. The net result from using Illustra™ Hot Start Master Mix is a more robust and reliable amplification of the target amplicons with increased yield when compared to conventional hot start master mix products.
Hot Start Master Mix was functionally tested:
To demonstrate prevention of primer-dimer formation during PCR amplification of numb locus fragment using oligonucleotide primers with 3-bp overlap at the
3, terminus when compared to reactions without Hot Start Activator protein.
To demonstrate that polymerase activity is blocked by 90% or greater when incubated for 4 h at 25 °C in the presence of DNA polymerase, dNTPs, and polymerase substrate compared to reactions without Hot Start Activator protein.
To ensure that Hot Start Master Mix is free of ribonucleases and double- and single-stranded DNA exonucleases and endonucleases. Standard assays were performed, and all of these activities were found to be negligible.
Storage: –20 °C
illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.
20 mM Tris-HCl (pH 8.6), 100 mM KCl, 3 mM MgCl2, 0.4 mM dNTPs (dATP, dCTP, dGTP, dTTP), 50 units/ml Taq DNA Polymerase, Hot Start Activator protein, stabilisers.