Dependable, consistent high-fidelity PCR results with every target DNA template, every time

• A complete, ready-to-use kit
• PCR amplification of difficult or high-GC templates
• Multiplex PCR compatible
• Robust amplification of targets up to 20 kb long
• High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
• Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence

How the FailSafe System Works
1. First, purchase the FailSafe PCR PreMix Selection Kit (catalog number FS99060). The FailSafe PCR PreMix Selection Kit is composed of:
FailSafe PCR Enzyme Blend (60 Units)
Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg+2 and Epicentre's proprietary PCR Enhancer (with Betaine).
2. Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme.
3. Analyze, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product.
4. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix indentified in the optimization experiments for all subsequent amplifications with that template/primer combination.
5. To reduce costs, purchase a FailSafe PCR System (Cat. No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favorite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.