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Specifications
- Description:Phosphate Assay Kit
- Size:500 assays
- Method:OD620 nm
- Sample:Enzyme reactions with free phosphate releases.
- Processing time:30 minutes
- Cat. no.:75878-188
- Supplier No.:POPB-500
Specifications
About this item
For sensitive and high-throughput phosphate determination.
- Very stable reagent
- High sensitivity and wide detection range (0.4 - 50 μM phosphate)
- Can be readily automated on HTS liquid handling systems
The PiBlue™ Phosphate Assay Kit is based on a proprietary formulation of the malachite green dye. The PiBlue™ reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged. Shelf Life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high-throughput screening of enzyme inhibitors.