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Specifications
- Description:Sorbitol Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological (e.g. blood), food, beverage and agriculture samples.
- Processing time:30 minutes
- Cat. no.:75878-168
- Supplier No.:ESBT-100
Specifications
About this item
For quantitative determination of D-sorbitol concentration.
- Linear detection range 5 - 1000 μM D-sorbitol.
- Convenient room temperature assay
- No 37°C incubator required
- Can be readily automated to test thousands of samples per day
The mitogen-activated protein kinase (MAPK/ERK) pathway plays a key role in cell proliferation, differentiation and migration
BioAssay Systems’ cell-based ELISA measures dually phosphorylated ERK1/2 in whole cells. This simple and efficient assay eliminates the need for cell lysate preparation and normalizes the signal to the total protein content. The assay is a useful tool to study the MAP kinase signaling pathway. In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1/2 phosphorylation (pERK) is measured using a HRP-conjugated antibody specific for phosphorylated ERK1/2.
Stimulation by mitogens eventually leads to phosphorylation of ERK1 (T202/Y204) and ERK2 (T185/Y187). The MAPK/ERK cascade presents many interesting drug targets for the development of cancer therapies.