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Specifications
- Description:Pyruvate Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Biological
- Processing time:20 minutes
- Cat. no.:75878-166
- Supplier No.:EPYR-100
Specifications
About this item
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
- Linear detection range 2 - 500 μM pyruvate for colorimetric assays and 0.2 - 50 μM for fluorimetric assays
- Convenient room temperature, high-throughput assay
Pyruvate is a key intermediate in cellular metabolic pathways
BioAssay Systems’ pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.
Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready. Procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.