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Specifications
- Description:Caspase Assay Kit
- Size:100 assays
- Method:FL400/490 nm
- Sample:Cell and tissue lysate
- Processing time:60 minutes
- Cat. no.:75877-968
- Supplier No.:DCS3-100
Specifications
About this item
For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.
- Safe
- Non-radioactive assay
- This homogeneous "mix-incubate-measure" type assay can be readily automated for processing thousands of samples per day
Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis
BioAssay Systems’ Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (λex/em = 400/490nm) is proportional to the caspase-3 activity.
Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction.