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Specifications
- Description:Glucosidase Assay Kit
- Size:100 assays
- Method:OD405 nm
- Sample:Biological
- Cat. no.:75877-964
- Supplier No.:DBGD-100
Specifications
About this item
For quantitative determination of beta-glucosidase activity and evaluation of drug effects on its metabolism.
- Linear detection range 2 to 250 U/L
- Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes
- Can be readily automated on high-throughput liquid handling instruments
β-Glucosidase is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose)
BioAssay Systems’ QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready. Procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery.