Transgenomic’s ICEme Kit is an in vitro assay that uses multiplexed PCR (MX PCR) followed by multiplexed ICE COLD-PCR (MX-ICP) for amplification and enrichment of mutations in KRAS Exons 2, 3 and 4, NRAS Exons 2, 3 and 4, BRAF Exons 11 and 15, PIK3CA Exons 9 and 20, EGFR Exons 12, 18, 19, 20 and 21.

• Non-Allele Specific
• Enriches ANY and ALL variants
• Suppresses wild-type while amplifying mutations
• Improves mutation detection sensitivity for any DNA sample
• Compatible with any sequencing platform
• LOD of mutations down to as low as 0.01%
• Can be incorporated easily into any laboratory work flow

Multiplexed ICE COLD-PCR (Improved and Complete Enrichment CO-amplification at Lower Denaturing temperature) technology preferentially enriches mutant DNA sequences in an excess of wild-type DNA through selective amplification. The result is up to a 500-fold increase in sensitivity in identifying mutations with the most precise sequence alteration detection rates available – down to 0.01%. The ICEme kits are compatible with DNA isolated from any sample type (tissue, blood, plasma, saliva, etc.) and are platform-independent, enabling analysis on ALL sequencing platforms (Sanger, NGS and ddPCR).