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Specifications
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Tag sequence:N-6 His tag
- Storage conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Protein synonyms:NAmPRTase|Nicotinamide phosphoribosyltransferase|Pre-B cell-enhancing factor|Nampt|Visfatin|PBEF1|PBEF|Pre-B-cell colony-enhancing factor 1
- Protein/peptide name:PBEF
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:57 kD
- Sequence:Met1-His491
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM HEPES, 150mM NaCl, pH8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. no.:75791-984
- Supplier No.:92-711
Specifications
About this item
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “visfatin”. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF- alpha, IL-1 beta , IL-16, and TGF- beta 1. PBEF also increases the production of IL-6, TNF- alpha, and IL-1 beta in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.
- Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.