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Human Recombinant Hemoglobin alpha (from E. coli)
Human Recombinant Hemoglobin alpha (from E. coli)
Catalog #: 75791-618
Supplier:  Prosci
CAS Number:  
Human Recombinant Hemoglobin alpha (from E. coli)
Catalog #: 75791-618
Supplier:  Prosci
CAS Number:  
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Specifications

  • Protein/peptide type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
  • Endotoxin content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Protein synonyms:
    HBA1|HBA-T3|Hemoglobin subunit alpha|Alpha-globin|Hemoglobin alpha chain|HBH
  • Protein/peptide name:
    Hemoglobin alpha
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular weight:
    16.7 kD
  • Sequence:
    Met1-Arg142
  • Formulation:
    Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Cat. no.:
    75791-618
  • Supplier No.:
    92-524

Specifications

About this item

Hemoglobin subunit alpha 1 (HBA1), also known as alpha2 beta 2, is a hetero-tetramer consisting of two alpha and two beta subunits held together by non-covalent interactions. Each subunit contains a heme group with an iron atom in the Fe2+ state. Cooperativity of Hemoglobin (Hb) in binding with O2 and allosteric regulatory binding properties with CO2, H+, Cl−, and 2,3-DPG (2,3-bisphosphoglycerate) are based on subunit interactions. HBA1 is the most common type of Hb in adult humans, which mediates the transport of oxygen and carbon dioxide in the blood. In recent years, Hb alpha and beta chains have been found co-expressed in alveolar cells, mesangial cells of the kidney, retinal ganglion cells, hepatocytes and neurons. Endothelial and peripheral catecholaminergic cells express exclusively the alpha chain, while macrophages present the beta chain only.

  • Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.

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