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Specifications
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme name:dUTP Pyrophosphatase
- Enzyme synonyms:Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial, dUTPase, dUTP Pyrophosphatase, DUT
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:17.7 kD
- Sequence:Met1-Asn164
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM PB, 150 mM NaCl,pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Used for biological assays
- Cat. no.:75790-770
- Supplier No.:92-088
Specifications
About this item
Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial (dUTPase) belongs to the dUTPase family
This recombinant protein can be used for biological assays. For research use only.
dUTPase exits as a homotrimer and is involved in nucleotide metabolism. dUTPase produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair.