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Specifications
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Mouse
- Size:0.05 mg
- Storage conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Biological activity:ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.<br />Specific Activity of 1.0 x 10<sup>8</sup> IU/mg.
- Protein synonyms:IL-1 Beta|Il1b|IL-1b|Interleukin-1 Beta|IL-1F2
- Protein/peptide name:IL1 beta
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:17.4 kD
- Sequence:Val118-Ser269
- Formulation:Lyophilized from a 0.2 um filtered solution of 50mM TrisHCl, 50mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
- Cat. no.:75788-788
- Supplier No.:91-032
Specifications
About this item
Interleukin-1 (IL-1) designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1 beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
This recombinant protein can be used for biological assays. For research use only.