To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Antibody type:Primary
- Antigen name:actinin, alpha 4
- Clonality:Polyclonal
- Gene ID:O43707
- Host:Rabbit
- Reactivity:Hamster,Pig,Human,Rat,Mouse
- Antigen symbol:ACTN4
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Size:50 ug
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:ACTN4
- Storage buffer:Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.
- Storage temperature:Stable for at least 1 year after receipt when stored at –20 °C.
- Concentration:250 ug/mL
- Shipping temperature:4 °C
- Immunogen:Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.
- Purification:Antigen affinity purified.
- Cat. no.:75793-484
- Supplier No.:36-344
Specifications
About this item
alpha-Actinin 4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).
Immunocytochemistry: (1:250- 1:1,600) . Immunohistochemistry: (1:250- 1:1,600) . Immunoprecipitation: (1:10) . Western Blot: (1:1,000-1:2,500) . Optimal conditions must be determined individually for each application.
Type: Primary
Antigen: ACTN4
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Hamster, Human, Mouse, Pig, Rat